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. 2015 Feb 10;6(10):7644–7656. doi: 10.18632/oncotarget.3223

Figure 4. Knockdown of HDAC9 induces cell cycle arrest in G1 phase.

Figure 4

(A) The cell cycle of HDAC9-knockdown U87 cells was analyzed by flow cytometry. (B) The effects of HDAC9 on the cell cycle of U87 cells. (C) The cell cycle of HDAC9-knockdown LN229 cells was analyzed by flow cytometry. (D) The effects of HDAC9 on the cell cycle of LN229 cells. (E, F) Western blot analysis of some cyclins and CDKs expression in HDAC9-knockdown cells; representative blots are shown. (G, H) Quantitative analysis of cyclins and CDKs expression in HDAC9-knockdown cells; GAPDH was used as a loading control; student's t-test was carried out. All data are shown as the mean ± SD, *p < 0.05, **p < 0.01.