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. 2015 Feb 10;6(10):7644–7656. doi: 10.18632/oncotarget.3223

Figure 7. Overexpression of TAZ in HDAC9-knockdown cells abrogates the effects induced by HDAC9 silencing.

Figure 7

(A, B) Western blot assay was used to characterize the expression of HDAC9 and TAZ in the TAZ-overexpressed HDAC9-knockdown U87 cells. (C) The effects of TAZ overexpression on the proliferation of HDAC9-knockdown U87 cells. (D, E) Western blot assay was used to characterize the expression of HDAC9 and TAZ in the TAZ-overexpressed HDAC9-knockdown LN229 cells. (F) The effects of TAZ overexpression on the proliferation of HDAC9-knockdown LN229 cells was measured by MTT assay. (G, H) The effects of TAZ overexpression on the cell cycle of HDAC9-knockdown cells. (I) The expression of cyclin E and CDK2 in TAZ-rescued HDAC9-knockdown cells were measured by western blot, GAPDH levels were showing as a loading control. (J) The effects of TAZ overexpression on colony formation of HDAC9-knockdown U87 cells was analyzed by soft agar assay. (K) The effects of TAZ overexpression on tumor formation in vivo of HDAC9-knockdown U87 cells. (L) Representative blots showing the expression of p-EGFR, EGFR, p-AKT, AKT, p-ERK1/2, ERK1/2 and p21 in the TAZ-overexpressed HDAC9-knockdown cells and xenograft tumor. All data are shown as the mean ± SD, *p < 0.05, **p < 0.01.