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. 2015 Mar 8;6(10):7774–7787. doi: 10.18632/oncotarget.3484

Figure 2. Change in CYP1B1 expression alters docetaxel resistance of RCC cells.

Figure 2

(A and B) After siRNA transfection, CYP1B1 protein expression was determined by Western blot (A) and enzyme activity was measured with P450-Glo assay (B) in A498 cells. *P < 0.05; **P < 0.01 (C and D) After CYP1B1 siRNA transfection, A498 cells were treated with docetaxel (5 μM) for 72 hrs. Apoptotic cell death was measured with annexin V-FITC/7-AAD staining (C) and cell survival was analyzed by MTS assay (D). *P < 0.05 (E and F) After plasmid transfection, CYP1B1 protein expression was determined by Western blot (E) and enzyme activity was measured with P450-Glo assay (F) in 786-O cells. *P < 0.05 (G and H) After CYP1B1 plasmid transfection, 786-O cells were treated with docetaxel (5 μM) for 72 hrs. Apoptotic cell death was measured with annexin V-FITC/7-AAD staining (G) and cell survival was analyzed by MTS assay (H). *P < 0.05; **P < 0.01.