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. 2015 Jun 15;142(12):2147–2162. doi: 10.1242/dev.121046

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Rab8a deletion affects Gpr177 transport to the plasma membrane but not ER export. (A) Quantification of Gpr177⁺ immunogold particle distributions in the ER, non-ER Golgi/vesicle and plasma membrane of Rab8a^+/+ and Rab8a^−/− Paneth cells (particles counted from 12 Rab8a^+/+ and nine Rab8a^−/− Paneth cells from three Rab8a^+/+ and two Rab8a^−/− mice, respectively). (B) The majority of Gpr177⁺ immunogold particles (arrows) were detected in the ER of Rab8a^+/+ and Rab8a^−/− Paneth cells. (C,D) Representative micrographs showing Gpr177⁺ immunogold particles in Golgi and lysosomes. Gold particles were detected in lysosomes (open arrowheads) in Rab8a^−/− (D) but not in Rab8a^+/+ (C). (E) Gpr177⁺ immunogold particles were frequently detected at apical or basolateral plasma membranes in wild-type Paneth cells. A significant reduction in plasma membrane-localized Gpr177⁺ particles was detected in Rab8a^−/− Paneth cells per unit length of plasma membrane. Data were collected for a total length of 312 µm plasma membrane in 12 Rab8a^+/+ Paneth cells and 328 µm plasma membrane in 14 Rab8a^−/− Paneth cells from three Rab8a^+/+ and two Rab8a^−/− mice, respectively. Arrowheads point to plasma membranes between a stem cell and a Paneth cell. lu, lumen. *P<0.05. Scale bars: 500 nm.