Table 1.

Overview of microscope components.

Component	Manufacturer	Part#	Specifications
Lasers	Cobolt AB, Sweden	MLD	405 nm, 80 mW, s-polarized
		MLD	445 nm, 50 mW, s-polarized
		Calypso	491 nm, 50 mW, s-polarized
		Fandango	515 nm, 50 mW, s-polarized
		Jive	561 nm, 50 mW, s-polarized
AOTF	AA Opto-Electronic, France	AOTFnC-400.650-TN	$>90\%$ diffraction efficiency, 3-nm resolution, low crosstalk between laser lines, high separation angle
Laser modulator	Qioptiq GmbH, Germany	LM 0202 VIS ADP	400 to 650 nm, $\lambda /2$-voltage (633 nm): 210 V
Pulse amplifier	Falco Systems, The Netherlands	WMA-300	$50\times$ amplification up to $\pm 150\mathrm{V}$, DC to 5-MHz signal bandwidth
Galvo scanner	Cambridge Technology, United States	6220H	Small angle step response $200\mu \mathrm{s}$
Objectives	Nikon, Japan	Plan Fluor EPI	$10\times 0.3\mathrm{NA}$, WD 17.5 mm, EFL 20 mm (excitation)
	Olympus, Japan	LMPLFLN20X	$20\times 0.4\mathrm{NA}$, WD 12 mm, EFL 9 mm (detection)
Motor stages	Physik Instrumente, Germany	C-863.11	DC servo-motor controller
		M-122	Travel range 25 mm, $0.1\mu \mathrm{m}$ resolution, max. velocity 20 mm/s
		M-116	Precision Rotation Stage, $2.5\mu \mathrm{rad}$, max. velocity $20\text{deg}/\mathrm{s}$
Camera	Hamamatsu, Japan	Orca Flash 4.0 v2.0	sCMOS sensor, $2048(\mathrm{H})\times 2048(\mathrm{V})$, cell dim.: $6.5\mu \mathrm{m}$, active area: $13.3\mathrm{mm}\times 13.3\mathrm{mm}$, 16 bit images
DAQ board	National Instruments, United States	NI PCIe-6353	AI: $1\mathrm{MS}/\mathrm{s}$ multichannel; 16-bit resolution, 10 V; AO: $2.86\mathrm{MS}/\mathrm{s}$, 16-bit resolution, 10 V; digital I/O lines (hardware-timed up to 10 MHz), 100 MHz max counter frequency
Workstation 1	Dell, United States	T7500	12 GB RAM, Intel Xeon Processor X5647 at 2.93 GHz, OS Windows 7 64 bit
Workstation 2	Dell, United States	T5600	8 GB RAM, Intel Xeon Processor E5-2620 at 2 GHz, OS Windows 7 64 bit