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. 2015 Mar 19;6(13):11447–11464. doi: 10.18632/oncotarget.3407

Figure 6. Reducing Cx43 expression increases migration in a spheroid migration assay.

Figure 6

(A) Western blot analysis shows successful expression of exogenous full-length Cx43 cDNA lacking the 3′UTR in U118-ShRNA6 and U118-ShRNA7 cells. Using densitometry we found the Cx43 expression in control and rescue cells to be 4 times higher than ShRNA6 and ShRNA7 cells (average of 3 Western blots). (B) Expression of Cx43 in the knockdown cells lowered migration level comparable to control cells. Scale bar = 100 μm. (C) Compared to control cells ShRNA6 and ShRNA7 cells increased migration 46% and 30%, respectively. The experiments were repeated three times with control (n = 81spheroids), ShRNA 6 (n = 81 spheroids), ShRNA 7 (n = 88 spheroids), ShRNA 6-Cx43 (n = 54 spheroids) and ShRNA 7-Cx43 (n = 54 spheroids). *p < 0.05 determined by One Way Anova method followed by Dunn's Method.