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AprA is present in and is secreted by vegetative cells. (A) Cells growing in shaking culture (vegetative cells), conditioned growth medium, and conditioned starvation buffer were electrophoresed on an SDS-polyacrylamide gel. A western blot of the gel was stained with affinity-purified anti-AprA antibodies. `aprA−/a15∷aprA' indicates aprA−/actin15∷aprA. (B) Growing cells were stained for AprA by immunofluorescence. Scale bar: 20 μm.
