Extended Data Figure 1. Immunoblots showing relative PrP^C expression levels in transgenic mice.

The provenance of each brain sample is designated above each lane and molecular markers are indicated on the left. The PRNP codon 127 (G, glycine, V, valine) or codon 129 (M, methionine, V, valine) genotypes of the transgenic mice are designated below. Transgenic mouse brains were analysed by enhanced chemiluminescence without proteinase-K digestion and equal amounts of total protein loaded in each well and probed with anti-PrP monoclonal antibody ICSM 35. Wild type human PrP expression levels of G¹²⁷M¹²⁹/G¹²⁷M¹²⁹ Tg35c and G¹²⁷V¹²⁹/G¹²⁷V¹²⁹ Tg152c mice are 2- and 6-fold higher respectively, than seen in 10 % (w/v) pooled human brain homogenate. Homozygous V¹²⁷M¹²⁹ Tg183 and Tg190 mice have 2-fold higher or equivalent PrP expression levels respectively compared to 10 % (w/v) pooled human brain homogenate.