Figure 5.

Meis1 and Pbx3 cooperate in leukemogenesis. (A) Meis1 heterodimerization with Pbx3 is rate-limiting for leukemogenesis. Kaplan-Meier survival curve of animals transplanted with syngenic primary cells transformed by either Hoxa9 (A9) or Hoxa9ΔPbx (A9Δ) in combination with either Meis1 (Meis), Pbx3 (Pbx), Meis1Δ (MeisΔ), Pbx3Δ (PbxΔ), or Meis1 + Pbx3 (Meis+Pbx) as indicated in the legend. Animals were transplanted on day 0 and observed for 6 months. Cohort size was initially five animals, with early deaths after radiation conditioning (< day 10) censored. (B) Spleen weight of euthanized animals succumbing to leukemia according to transplantation cohorts. (C) PCR analysis of white blood cell-derived genomic DNA from animals surviving 180 days after transplantation. A primer pair specifically detecting the presence of transduced Hoxa9/Hoxa9ΔPbx cDNA was used to probe for the presence of integrated provirus in genomic DNA of white blood cells isolated from recipients. St = 100bp standard.