Fig. 2.

Example of images generated from the AF calculation method. (a) Original image generated from section 2.3.1 of UPN251 cells. (b) Image ‘Drawing of X.JPG’ generated after analysis step 7, represents the cells counted from the analysis of original image. (c) Image ‘Drawing of X.JPG’ after accuracy step 5. This is the image that is used in the overlay onto the original image, to check for accuracy of the generated count versus the cells present in original image. (d) The result of the merged images (‘a’ and ‘c’) after completion of accuracy steps. In this case the count looks accurate as each cell is labelled with red numbering.