TABLE I.
State of art in microfluidic diffusion-based gradient devices and their biological applications.
| Biological application | Cell type | Stimuli | Microfluidic platform | Reference |
|---|---|---|---|---|
| Cancer chemotaxis | Breast cancer cells (MDA-MB-231) | EGF | Microfluidic cell-motility-based sorting device, compartmented microfluidic device | 37, 39, and 40 |
| Prostate cancer cell line (PC-3) | EGF | PDMS microfluidic device | 41 and 42 | |
| Migration of smooth muscle cells | Primary smooth muscle cells (SMC) | Platelet-derived growth factor (PDGF) | PDMS microfluidic device | 43 |
| Migration of fibroblast cells | Connective tissue-derived fibroblast cells | EGF | PDMS microfluidic device | 44 |
| Stem cell differentiation | Rat adipose-derived stem cells (ASCs) | EGF | “Y” shaped microfluidic gradient device | 46 |
| Neutrophil chemotaxis | HL-60 cells (human promyelocyticleukemia cells) | Chemoattractant f-MLP | Hydrogel based microfluidic device | 45 |
| Dendritic cell chemotaxis | Bone marrow dendritic cells | mCCL21 and mCCL19 chemokines | Agarose-based microfluidic chemotaxis device | 51 |