Table 3. Circular Dichroism and Thermal Denaturation Parametersa.
sample | −[θ]222 nm (deg cm2 dmol−1 res−1) | [θ]222 nm/[θ]208 nm | Tm (°C) | ΔHcalb (kcal mol−1) | ΔHcal/ΔHvan't Hoff |
---|---|---|---|---|---|
α3Dc | 25213 (306) | 1.01 | 89.6 (0.3) | 49.9 (4.6) | 1.2 (0.3) |
α3DIV | 29231 (672)c | 1.03 | 64.4 (0.6)d | 50.0 (0.2) | 2.4 (0.4) |
60.2 (0.1)c | 44.9 (1.2) | 1.9 (0.2) | |||
Hg-α3DIVc | 27589 (421) | 1.51 | 84.0 (1.7) | 65.1 (3.5) | 6.7 (2.4) |
Pb-α3DIVc | 26940 (2420) | 1.01 | 83.4 (3.3) | 59.2 (6.9) | 4.5 (2.3) |
Cd-α3DIVc | 27600 (487) | 1.07 | 78.1 (0.7) | 52.7 (4.3) | 4.2 (0.5) |
All the averaged values were determined from triplicate or duplicate experiments.
Calculated excess heat capacity.
pH 8.2.
pH 7.0.