Table 1. Catalytic efficiencies of FadD mutants.

Catalytic efficiency (K_cat/K_m) of wild-type FadD and the indicated mutants as measured by AMP production assay.

FadD variant	Octanoate Kcat/Kma	Oleate Kcat/Kma
WT	0.10 ± 0.07	1.70 ± 0.94
Q338R	0.25 ± 0.13	1.98 ± 1.07
H376R	0.10 ± 0.06	1.16 ± 0.54
V4F, W5L	0.10 ± 0.05	0.46 ± 0.20
F447S	0.05 ± 0.02	0.74 ± 0.50
V451A	0.35 ± 0.28	1.46 ± 1.01
D372G	0.14 ± 0.05	1.09 ± 0.65
Y9H	0.20 ± 0.07	1.38 ± 0.80

Notes.

^a(M⁻¹∗s⁻¹∗10⁵) Values are indicated ± standard deviation.