. 2015 Apr 14;6(14):12080–12093. doi: 10.18632/oncotarget.3813

Table 1. A Table summarizing cell cycle protein expression in mouse MTC tumors, human MTC-SK cells and human MTC tissues.

	Mouse model		Human cell line	Patient tissues
	Arrested proliferating MTC	vs	kd-Cdk5-transfected cells vs control cells	Sporadic MTC control thyroid	vs	Hereditary MTC vs control thyroid
Cdk2	↓		↓	↑		↓
Cdk4	-		↓	-		-
Cdk6	-		↓	-		-
cyclin A1	↓		↓	-		-
cyclin B1	↓		↓	n.d.		n.d.
cyclin D1	-		n.d.	↑		-
cyclin E2	↓		↓	-		-
p15^INK4b	↓		↓	↑		↑
p16^INK4a	-		-	↑		↑
p18^INK4c	↓		-	↑		-
p19^INK4d	↓		↓	↑		-
p21^CIP/WAF1	↓		↓	(↑)		-
p27^KIP1	-		-	-		-

To determine the role of CDK5 activity in the expression of cell cycle regulators, we assessed the changes in protein level of cyclins, CDKs, and CKI in mouse tumors and in MTC-SK cells following blockade of CDK5 activity. We have indeed previously demonstrated that CDK5 activity is reduced in arrested tumors by stopping p25-GFP transgene expression and in MTC-SK cells by overexpressing a kinase-dead CDK5 construct [10]. The changes in expression of cell cycle regulators in human MTC tumors compared to normal thyroid tissues are also reported.