Figure 5. Knockdown of PAQR3 in MDA-MB-231 cells enhances cell growth and migration.

(A) The mRNA level of PAQR3 in MDA-MB-231 cells infected with lentivirus containing mock or PAQR3-specific shRNA as detected by quantitative RT-PCR. (B) Cell proliferation rate of these cells were determined by MTT assay at the indicated time point. (C) Colony formation assay was performed in these cells that were stained by crystal violet. (D) A scratched-wound healing assay was performed with these cells, followed by photography at 0 h and 24 h after the scratch. The lower panel denotes statistical analysis. (E) Transwell assay was performed with these cells, followed by photography and counting. The statistical results are shown in the lower panel. All the data are shown as mean ± SD and ** for P < 0.01.