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. 2015 Mar 12;6(12):10045–10059. doi: 10.18632/oncotarget.3541

Figure 5. Transient overexpression of miR145 downregulated cell growth and migration and increased apoptosis through downregulation of VASN expression in HepG2 cells.

Figure 5

(a) The expression level of VASN mRNA was down regulated after transient transfection of miR145 mimics into HepG2 cells by real-time PCR. (b) The expression level of VASN protein was down regulated after transient transfection of miR145 mimics into HepG2 cells by western blot. (c) Overexpression of miR145 inhibited HepG2 cell growth by MTS assay. (d) Overexpression of miR145 mimics inhibited HepG2 cell migration by transwell chamber migration assay. (e) Overexpression of miR 145 mimics in HepG2 inhibited migration in a scratch wound assay. Upper: A scratch was created with a 200 ul-pipette tip and was photographed. Lower: Photographs at 36h after scratching. (f) Overexpression of miR145 induced HepG2 cell apoptosis.