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. 2015 Mar 19;6(12):10498–10509. doi: 10.18632/oncotarget.3403

Figure 2. Increased cycling and reduced quiescent HSC fractions in Cbl/Cbl-b DKO mice.

Figure 2

(A–B) LTC-IC assay – cell counts after initial co-culture (A), and subsequent colony-forming assays (B) Results from three experiments are pooled; 2 replicates in each experiment. (C) Serial colony-forming assay. Results from three experiments are pooled; 3 replicates in each experiment. (D–F) Cell cycle analysis. Representative FACS plots are shown in (D) Quantification of cell cycle phases for total LSKs (E); LT-HSC, ST-HSC and MPP subpopulations (F) Data from at least three experiments are pooled and shown as mean ± SD. (G) qRT-PCR of p57, p27 and p21 mRNA in LT-HSCs normalized using GAPDH. Data from four independent repeats are pooled and shown as mean ± SD (*p < 0.05).