Table 4.
Sequences of primers for adipose or liver tissue. Beta-2 microglobulin (B2M), C/EBP homologous protein (CHOP), collagen-α1 (Col1a1) fatty acid synthase (FASN), growth arrest and DNA damage inducible protein 34 (GADD34), glucose regulated protein 78 (GRP78), lipoprotein lipase (LPL), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element-binding protein-1c (SREBP-1c), transforming growth factor-β (Tgfb1), vascular endothelial growth factor (VEGF) and spliced X box binding protein-1 (XBP-1)
| Target Gene | Sequence |
|---|---|
| B2M | F-CGGTCGCTTCAGTCGTCAG |
| R-ATGTTCGGCTTCCCATTCTCC | |
| CHOP | F-CGCTCTCCAGATTCCAGTCAG |
| R-GTTCTCCTGCTCCTTCTCCTT | |
| Col1a1 | F-ATGTATCACCAGACGCAGAAG |
| R-TCTTGAGGTTGCCAGTCTGC | |
| FASN | F-AGACTACAGACGACAGCAACC |
| R-CTCTCAGACAGGCACTCAGC | |
| GADD34 | F-CAGAAGATGACACAGAAGAGG |
| R-TCTCTCCTGGTAGACAACGC | |
| GRP78 | F-GAGGCGTATTTGGGAAAGAAGG |
| R-GCTGCTGTAGGCTCATTGATG | |
| LPL | F-TTCCAGCCAGGATGCAACA |
| R-GGTCCACGTCTCCGAGTCC | |
| PPARγ | F-GCG GTG AAC CAC TGA TAT TCA GGA CA |
| R-TCCGAAGTTGGTGGGCCAGA | |
| Srebp-1c | F-TGGTGGGCACTGAAGCAAAG |
| R-CACTTCGTAGGGTCAGGTTCTC | |
| Tgfb1 | F-TGGACACACAGTACAGCAAGG |
| R-GTAGTAGACGATGGGCAGTGG | |
| Vegf | F-TGTGCAGGCTGCTGTAACGATGAA |
| R-ATGTGCTGGCTTTGGTGAGGTTTG | |
| XBP-1 | F-GGCATTCTGGACAAGTTGG |
| R-GAAAGGGAGGCTGGTAAGG |