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. 2015 Jan 28;10(12):2143–2157. doi: 10.4161/auto.36438

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© 2014 The Author(s). Published with license by Taylor & Francis

© Benjamin Cull, Joseane Lima Prado Godinho, Juliany Cola Fernandes Rodrigues, Benjamin Frank, Uta Schurigt, Roderick AM Williams, Graham H Coombs, and Jeremy C Mottram

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Visualization of autophagosome formation and fusion with lysosomes. (A) L. major promastigotes coexpressing GFP-ATG8 and RFP-SQL were imaged by fluorescence microscopy. Panels at lower left show enlarged images of cup-shaped autophagosomes. Panel on far right shows a section of a TEM of a stationary phase parasite. Arrows mark glycosomes that are being surrounded by autophagosomes. Arrowheads in TEM image show double-membrane forming around glycosome (marked with arrow). (B) Stationary phase L. major coexpressing GFP-ATG8 and proCPB-RFP. Enlarged images of autophagosome-lysosome fusion are displayed in insets. White arrow indicates autophagosome; yellow arrowhead marks lysosome containing GFP-ATG8 and proCPB-RFP. Images show a single slice from a 3-μm Z-stack. Scale bar = 5 μm.