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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biomaterials. 2015 Jun 20;64:88–97. doi: 10.1016/j.biomaterials.2015.06.038

Fig. 2.

Fig. 2

Preconditioning using sb203580 enhanced chondrogenesis of dECM expanded hSDSCs. Human SDSCs expanded on either dECM or Plastic for one passage (8 days) with or without preconditioning of sb203580 were followed by a 35-day chondrogenic induction in a pellet system. (A) Pellets were photographed before histology; Alcian blue (Ab) was used to stain sulfated GAGs and immunohistochemical staining (IHC) was for Col II. (B) Biochemical analysis was used for DNA and GAG amounts in chondrogenic pellets. Cell proliferation and viability were evaluated using DNA ratio (DNA amount at days 35 and 14 adjusted by that at day 0). Chondrogenic index was evaluated using ratio of GAG to DNA. Data are shown as average ± SD for n = 4. * p < 0.05 suggests a statistically significant difference. NS indicates no significant difference.