Abstract
Plant defense responses are induced when the products of disease-resistance genes and pathogen avirulence genes interact. We report here the effects of expressing the Cladosporium fulvum avirulence Avr9 gene product in a tomato line containing the Cf-9 disease-resistance gene. A synthetic Avr9 gene was constructed to produce constitutive high-level expression of AVR9 peptide in the plant apoplast. Avr9 expression in Cf-9-containing tomato lines is lethal, but cell death is developmentally regulated, in that necrosis is not visible until 10 days after planting seed. Plant lines lacking Cf-9 and expressing Avr9 remain healthy. The synthetic Avr9 gene exhibited the same specificity of action as the authentic C. fulvum Avr9 gene. Our results have significant implications for strategies using the gene combination Avr9/Cf-9 to engineer plants with enhanced disease resistance.
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Selected References
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- Bradley D. J., Kjellbom P., Lamb C. J. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992 Jul 10;70(1):21–30. doi: 10.1016/0092-8674(92)90530-p. [DOI] [PubMed] [Google Scholar]
- Chapman S., Lindbeck A. G., Dawson W. O. The structure of viral coat protein and its in disease. Trends Microbiol. 1993 Oct;1(7):280–283. doi: 10.1016/0966-842x(93)90052-s. [DOI] [PubMed] [Google Scholar]
- Cornelissen B. J., Horowitz J., van Kan J. A., Goldberg R. B., Bol J. F. Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group. Nucleic Acids Res. 1987 Sep 11;15(17):6799–6811. doi: 10.1093/nar/15.17.6799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallie D. R., Sleat D. E., Watts J. W., Turner P. C., Wilson T. M. The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo. Nucleic Acids Res. 1987 Apr 24;15(8):3257–3273. doi: 10.1093/nar/15.8.3257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halliwell B., Gutteridge J. M. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol. 1990;186:1–85. doi: 10.1016/0076-6879(90)86093-b. [DOI] [PubMed] [Google Scholar]
- Jones J. D., Shlumukov L., Carland F., English J., Scofield S. R., Bishop G. J., Harrison K. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res. 1992 Nov;1(6):285–297. doi: 10.1007/BF02525170. [DOI] [PubMed] [Google Scholar]
- Joosten M. H., Cozijnsen T. J., De Wit P. J. Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene. Nature. 1994 Jan 27;367(6461):384–386. doi: 10.1038/367384a0. [DOI] [PubMed] [Google Scholar]
- Keen N. T. Gene-for-gene complementarity in plant-pathogen interactions. Annu Rev Genet. 1990;24:447–463. doi: 10.1146/annurev.ge.24.120190.002311. [DOI] [PubMed] [Google Scholar]
- Long S. R., Staskawicz B. J. Prokaryotic plant parasites. Cell. 1993 Jun 4;73(5):921–935. doi: 10.1016/0092-8674(93)90271-q. [DOI] [PubMed] [Google Scholar]
- Van den Ackerveken G. F., Van Kan J. A., De Wit P. J. Molecular analysis of the avirulence gene avr9 of the fungal tomato pathogen Cladosporium fulvum fully supports the gene-for-gene hypothesis. Plant J. 1992 May;2(3):359–366. doi: 10.1111/j.1365-313x.1992.00359.x. [DOI] [PubMed] [Google Scholar]
- Van den Ackerveken G. F., Vossen P., De Wit P. J. The AVR9 race-specific elicitor of Cladosporium fulvum is processed by endogenous and plant proteases. Plant Physiol. 1993 Sep;103(1):91–96. doi: 10.1104/pp.103.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vera-Estrella R., Blumwald E., Higgins V. J. Effect of Specific Elicitors of Cladosporium fulvum on Tomato Suspension Cells : Evidence for the Involvement of Active Oxygen Species. Plant Physiol. 1992 Jul;99(3):1208–1215. doi: 10.1104/pp.99.3.1208. [DOI] [PMC free article] [PubMed] [Google Scholar]