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. 2015 Aug 1;116:123–134. doi: 10.1016/j.neuroimage.2015.05.012

Table S8.

Summary of main effects and interactions of SW half wave polarity, sleep dependent and circadian factors on the studied SW parameters as measured during the forced desynchrony.

SW parameter Segment Effect DF F value P value Cohen's f2
Incidence Polarity 1 0.04 ns
Sleep dependent 2 1024.41 < 0.0001**** 17.03L
Circadian 5 44.51 < 0.0001**** 0.65L
Polarity ∗ Circadian 5 0.01 ns
Polarity ∗ Sleep dependent 2 0.07 ns
Sleep dependent ∗ Circadian 10 7.34 < 0.0001**** 0.16M
Amplitude Polarity 1 8.64 0.004* 0.08S
Sleep dependent 2 440.31 < 0.0001**** 7.19L
Circadian 5 23.43 < 0.0001**** 0.41L
Polarity ∗ Circadian 5 0.08 ns
Polarity ∗ Sleep dependent 2 0.08 ns
Sleep dependent ∗ Circadian 10 4.6 < 0.0001**** 0.10S
Duration Initial Polarity 1 0.47 ns
Sleep dependent 2 32.11 < 0.0001**** 0.50L
Circadian 5 11.3 < 0.0001**** 0.18M
Polarity ∗ Circadian 5 0.18 ns
Polarity ∗ Sleep dependent 2 0.34 ns
Sleep dependent ∗ Circadian 10 4.13 < 0.0001**** 0.08S
Final Polarity 1 21.2 < 0.0001**** 0.16M
Sleep dependent 2 11.84 < 0.0001**** 0.20M
Circadian 5 8.97 < 0.0001**** 0.15S
Polarity ∗ Circadian 5 0.19 ns
Polarity ∗ Sleep dependent 2 2.92 ns
Sleep dependent ∗ Circadian 10 3.69 < 0.0001**** 0.07S
Mean slope Initial Polarity 1 0.47 ns
Sleep dependent 2 123.33 < 0.0001**** 1.94L
Circadian 5 35.57 < 0.0001**** 0.54L
Polarity ∗ Circadian 5 0.2 ns
Polarity ∗ Sleep dependent 2 0.03 ns
Sleep dependent ∗ Circadian 10 3.76 < 0.0001**** 0.07S
Final Polarity 1 14.22 0.0003*** 0.12S
Sleep dependent 2 94.34 < 0.0001**** 1.59L
Circadian 5 22.65 < 0.0001**** 0.41L
Polarity ∗ Circadian 5 0.57 ns
Polarity ∗ Sleep dependent 2 2.68 ns
Sleep dependent ∗ Circadian 10 2.65 0.0037* 0.05S
Maximum slope Initial Polarity 1 12.6 0.0006** 0.12S
Sleep dependent 2 197.96 < 0.0001**** 3.16L
Circadian 5 40.39 < 0.0001**** 0.61L
Polarity ∗ Circadian 5 0.22 ns
Polarity ∗ Sleep dependent 2 0.55 ns
Sleep dependent ∗ Circadian 10 3.58 0.0001**** 0.07S
Final Polarity 1 5.9 0.0168 0.05S
Sleep dependent 2 252.94 < 0.0001**** 4.14L
Circadian 5 33.58 < 0.0001**** 0.58L
Polarity ∗ Circadian 5 0.56 ns
Polarity ∗ Sleep dependent 2 1.26 ns
Sleep dependent ∗ Circadian 10 2.67 0.0035* 0.05S

We assessed the effect of polarity on the studied SW parameters in addition to the circadian and sleep-dependent factors. We found that factor ‘polarity’ had an independent significant effect on most SW parameters except incidence, duration and mean slope of the initial segment and the maximum slope of the final segment of SW half-waves. However, overall the effect size of polarity was smaller as compared to the sleep-dependent and the circadian regulation, which yielded strong significance and no significant interaction with polarity.

The polarity factor includes the SW parameters for the negative and positive half-waves. The sleep-dependent factor includes thirds of the total sleep period (9 h 20 m). The circadian factor comprised 6 ∗ 60° bins. The Segment variable indicates the descending (initial) or the ascending (final) phase of the slow wave (SW) negative half waves. Degree of freedom (DF), F values, P values, effect size (Cohen's f2) of main effects, and interactions are indicated for each studied variables as returned from mixed model analyses of variances (*P < .005, **P < .001, ***P < .0005, ****P < .0001). Superscripts following effect size values indicate the magnitude of the effects size [small (S): 0.02–0.15, medium (M): 0.15–0.35, large (L): > 0.35]. P values and effect sizes for non-significant effects are not indicated. Non-significant trends (< 0.05) are indicated.