Figure 1.

Loss of U₃₄ Modifications Leads to Codon-Specific Ribosome Pausing in Yeast and Nematodes

(A) Pathways for wobble uridine (U₃₄) modification in the eukaryotic cytoplasm.

(B) Codon-specific changes in A-site ribosome occupancy in S. cerevisiae strains with aberrant U₃₄ modification in comparison to WT (mean ± SD; n = 3). Codons cognate for tRNAs with mcm⁵s²U₃₄ (yellow), mcm⁵U₃₄ (blue), and ncm⁵U₃₄ (cyan) are boxed. Symbol size reflects the relative frequency of each codon within the A site in WT (small, <0.005; medium, 0.005–0.015; large, >0.015).

(C) Codon-specific changes in A-site ribosome occupancy upon U₃₄ thiolation loss in C. elegans compared to WT (n = 2). Codons cognate for tRNAs with mcm⁵s²U₃₄ are boxed in yellow; symbol size as in (B).

See also Figures S1 and S2.