Skip to main content
. Author manuscript; available in PMC: 2015 Jul 19.
Published in final edited form as: JOP. 2013 Mar 10;14(2):176–186.

Figure 1.

Figure 1

Experimental workflow. 1) Pancreatic fluid was collected via ePFT. 2) Samples were centrifuged to remove any particulates. 3) Pancreatic fluid was precipitated with TCA or subjected to ultracentrifugation at 200,000 g for 2 h. 4) The pellet was solubilized in LDS Laemmli loading buffer. 5) Proteins were fractionated by SDS-PAGE. 6) Proteins were in-gel typically digested. 7) Resulting peptides were subjected to LC-MS/MS analysis. 8) Proteins were identified via database searching using Proteome Discoverer (Thermo Scientific, Waltham, MA, USA) and Scaffold 3 (Proteome Software Inc., Portland, OR, USA) software.