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. 1994 Oct 25;91(22):10640–10644. doi: 10.1073/pnas.91.22.10640

Gene regulation by temperature-sensitive p53 mutants: identification of p53 response genes.

L Buckbinder 1, R Talbott 1, B R Seizinger 1, N Kley 1
PMCID: PMC45077  PMID: 7938006

Abstract

The ability of the p53 protein to act as a sequence-specific transcriptional activator suggests that genes induced by p53 may encode critical mediators of p53 tumor suppression. Using a tetracycline-regulated p53 expression system and cDNA library subtraction procedure, we identified several p53-induced gene transcripts in human Saos-2 osteosarcoma cells that are novel on the basis of their size, regulation, and low abundance. Wild-type p53-dependent induction of these transcripts was observed in cells that are growth arrested by p53, as well as in cells that undergo apoptosis upon expression of an inducible wild-type p53 transgene. These results show that p53 activates the expression of numerous response genes and suggest that multiple effectors may play a role in mediating cellular functions of p53.

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