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. Author manuscript; available in PMC: 2015 Jul 20.
Published in final edited form as: Oncogene. 2013 Sep 2;33(25):3307–3315. doi: 10.1038/onc.2013.291

Fig. 3. In situ RNA FISH Analysis of Dleu2 and Pax5 mRNA levels.

Fig. 3

Cell were grown on poly L-lysine coated coverslips and hybridized with single molecule FISH probes. Dleu2 probes labeled were with TMR and Pax5 probes were labeled with Alexa 594. The z-stacks obtained from each fluorescence channel were merged and coded to represent Pax5 as red and Dleu2 as green. Each spot represent single mRNA molecule [65]. The right panel is the merge of both channels and the chart represents the mean values from multiple analysis ± SEM.