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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Anesthesiology. 2015 Aug;123(2):377–388. doi: 10.1097/ALN.0000000000000742

Figure 3. Isoflurane does not impact lung inflammation after nebulized endotoxin, mechanical ventilation, or the combination of both injuries.

Figure 3

Total bronchoalveolar lavage (BAL) inflammatory cells (A) and neutrophils (B) were increased following injury with lipopolysaccharide (LPS, n=3) and LPS followed by mechanical ventilation to induce ventilator induced lung injury (LPS/VILI, n=7) compared to control mice treated with nebulized phosphate buffered saline (PBS, n=2) or PBS treatment followed by mechanical ventilation (PBS/VILI, n=8), but were not affected by isoflurane treatment. Interleukin-6 (IL-6) levels in BAL fluid (C) increased following LPS/VILI (n=7) compared to PBS/VILI (n=7) but were not altered by isoflurane treatment. Staining for Gr-1 revealed increased neutrophils in animals subjected to LPS/VILI compared to either injury alone but there was no significant change in neutrophil infiltration with isoflurane (D).