Figure 2.

Protoplast transient dual-luciferase assays show maize-TGA is a transcriptional repressor. (A) Diagram showing effector construct components. (B) Diagram showing reporter construct components. (C) Effectors 1–5 were cotransformed with both reporters into maize protoplasts with and without the activation control (effector 6). Dual-luciferase assay results are shown in relative light units (RLU), y-axis, (±SE; n = 6). Maize-TGA1 (effector 2) shows stronger repression than the repression control (effector 5).