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. 1994 Nov 8;91(23):11032–11034. doi: 10.1073/pnas.91.23.11032

Isolation of the yeast histone octamer.

Y Lorch 1, R D Kornberg 1
PMCID: PMC45160  PMID: 7972003

Abstract

Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt extracts of nuclei or chromatin to near homogeneity. The histone octamer purified in this way was fully active in reconstitution of nucleosomes.

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Selected References

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