Fig. 7.

Role of DNA-PK and prohibitins on the anti-apoptosis activities of BCL-xL and E1B-19K. A. Depletion of DNA-PK and prohibitins. A549 cells were infected with lentiviral vectors (pLKO-puro) that express shRNAs targeted against DNA-PKcs, prohobitin 1 and prohibitin 2 and selected with puromycin. B. Effect on BCL-xL activity. The acutely depleted cells were pooled and infected with the recombinant viruses (50 pfu/cell) and the cell death assay was performed as outlined in Fig. 2B in the presence and absence of etoposide. The experiment was repeated three times and the statistical differences were assessed as in Fig. 3. P-value was determined by comparing the 3 cell lines in the presence and absence of etoposide. Significance was accepted at a value of **p<0.01 and ***p<0.005.