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Pre-exposure with inhibitor of Src kinase, PP2 (20 µM) and PI3-Kinase inhibitors wortmannin (30 nM) and LY294002 (20 µM) for 30 min significantly down-regulated p-eNOSSer1177 (A); and p-AktSer473 (B) protein expression; and (C) total nitrate/nitrite (NOx) level induced by AVLE (10 µg/mL, 30 min) in HUVECs. Results are means ± SEM (n = 3–5). * p < 0.05 compared to control, # p < 0.05 compared to AVLE.
