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. 2015 Jul 17;7(7):3954–3973. doi: 10.3390/v7072807

Figure 5.

Figure 5

Effect of ncRNA dosage on cytokine induction in total swine PBMCs or after pDCs-enrichment. (A). PBMCs were transfected with S, IRES or 3′NCR at 5, 20 or 100 μg/mL and the IFN-α, TNF-α, IL10 and IL12 levels were measured by ELISA. The data shown are the ratios of the values obtained at 5 or 100 μg/mL for the indicated cytokine relative to those obtained at 20 μg/mL with the corresponding RNA; (B) Total PBMCs or PBMCs enriched in pDCs by negative selection were transfected with S, IRES or 3′NCR (at 5 or 20 μg/mL) or stimulated with 10 μg/mL ODN. Data indicate levels of IFN-α, TNF-α, IL-10 and IL-12 in pDCs-enriched PBMCs transfected with the ncRNAs relative to those in total PBMCs. IFN-α levels in total or pDCs-enriched PBMCs stimulated with ODN are also shown.