Correction: Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana

The second panel from the bottom of the left side of Fig 1D, is missing. The authors have provided a corrected version here.

Fig 1. Protein-protein interactions between IDM1, IDM2, IDL1 and MBD7.

(A) Yeast two-hybrid assays. Yeast cells carrying different fusion protein combinations are listed on the left. Yeast cells expressing the indicated proteins from the pGBK-T7 (BD) and pGAD-T7 (AD) vectors were plated onto medium lacking Leu and Trp (SD-LT) (left) or medium lacking Leu, Trp, Ade and His (SD-LTHA) (right). (B) Pull-down assays showing that IDM2, IDL1 and MBD7 interact with each other. (C) Split-luc assays showing that IDL1 and MBD7 can interact with IDM2 in N. benthamianaleaves. Three biological replicates were performed, and similar results were obtained. (D) Co-immunoprecipitation of MBD7 with IDM2 or IDL1 in tobacco leaves. MYC-tagged IDM2 and GFP-tagged IDM1 were transiently expressed in N. benthamiana leaves. Anti-GFP was used for immunoprecipitation (IP); anti-MYC and anti-GFP were used for immunoblotting; Input, total protein before immunoprecipitation. Transgenic plants expressing MBD7-Myc or IDM1-HA-YFP under their native promoters and their F1 offspring were used for co-IP.