VOLUME 286 (2011) PAGES 3531–3539
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The Km value of 36 ± 9 μm reported for the reaction of wild type dehydroquinate dehydratase from Gram-positive Clostridium difficile (wild type cd-DHQD) in Table 2 was not correct. The correct Km value is 171 ± 36 μm. This correction does not affect the interpretation of the results or the conclusions of this work.
TABLE 2.
Kinetic characterization of seDHQD and cdDHQD
Errors were calculated by fitting of the kinetic data to the Michaelis-Menten equation and expressed as ± S.D.
| kcat | Km | kcat/Km | |
|---|---|---|---|
| s−1 | μm | s−1 μm−1 | |
| seDHQD wild type | 210 ± 5 | 21 ± 3 | 10 ± 1 |
| seDHQD K170M | 0.015 ± 0.007 | 33 ± 4 | 4.5 × 10−4 ± 2.3 × 10−4 |
| cdDHQD wild type | 125 ± 4 | 171 ± 36 | 0.7 ± 0.15 |