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. 1996 Aug 1;15(15):3943–3950.

Phosphorylation of Drosophila Jun by the MAP kinase rolled regulates photoreceptor differentiation.

F A Peverali 1, A Isaksson 1, A G Papavassiliou 1, P Plastina 1, L M Staszewski 1, M Mlodzik 1, D Bohmann 1
PMCID: PMC452112  PMID: 8670899

Abstract

Drosophila Jun (D-Jun) is a nuclear component of the receptor tyrosine kinase/Ras signal transduction pathway which triggers photoreceptor differentiation during eye development. Here we show that D-Jun is a substrate for the ERK-related Drosophila MAP kinase Rolled, which has previously been shown to be a part of this pathway. A D-Jun mutant that carries alanines in place of the Rolled phosphorylation sites acts as a dominant suppressor of photoreceptor cell fate if expressed in the eye imaginal disc. In contrast, a mutant in which the phosphorylation sites are replaced by phosphate-mimetic Asp residues, as well as a VP16-D-Jun fusion protein, can promote photoreceptor differentiation. These data implicate Jun phosphorylation in the choice between neuronal and non-neuronal fate during Drosophila eye development.

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