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. 2015 Jul 31;4:e07420. doi: 10.7554/eLife.07420
Reagent Type Manufacturer Catalog # Comments
D-2-HG Reagent Sigma–Aldrich H8378
L-2-HG Reagent Sigma–Aldrich 90790
Sf9 cells Cells ATCC CRL-1711 Original unspecified
Shrimp alkaline phosphatase Reagent New England Biolabs MO371S
T4 polynucleotide kinase Reagent Sigma–Aldrich KEM0006
DNase I Reagent Sigma–Aldrich AMPD1
Phosphodiesterase I Reagent Sigma–Aldrich P3243
PEI-cellulose TLC plate Material Sigma–Aldrich Z122882
FLAG-TET2-CD viral particles Virus Provided by the original authors
Anti-Flag M2 antibody agarose affinity gel Reagent Sigma–Aldrich A2220
Flag peptide Reagent Sigma–Aldrich F4799
α-KG Reagent Sigma–Aldrich 75,892
GenElute PCR Clean-Up Kit Kit Sigma–Aldrich NA1020-1KT Replaces Qiagen cat no. 28304
[γ-32]ATP Reagent Perkin Elmer BLU502H/NEG502H
MspI methyltransferase Reagent NEB M0215L
MspI restriction endonuclease Reagent NEB R0106T
DNA duplex oligonucleotide substrate oligo Integrated DNA Technologies custom 5′-GTGTTCTTTCAGCTCCGGTCACGCTGACCAGC-3′ as a duplex oligo, HPLC purified at 1 umole scale maybe higher depending on recovery
M13-F primer oligo Integrated DNA Technologies CCAGTCACGACGTTGTAAAACG
M13-R primer oligo Integrated DNA Technologies CCAGTCACGACGTTGTAAAACG
JumpStart REDTaq DNA Polymerase Reagent Sigma D8189-50UN
dNTP mix 10 mM Reagent Sigma D7295-.2 ML
BlueView TAE buffer Buffer Sigma T8935-1L
Molecular biology grade water Reagent Sigma W4502-1L