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Variable-temperature 31P spectra of static DMPC/DHPC (3 : 1) bicelles with and without M2. (a) Bicelles without protein. (b) Bicelle with M2TM. (c) Bicelle with M2TM-AH. M2TM-AH creates a temperature-independent isotropic 31P peak, indicating the formation of a high-curvature membrane phase. The other 31P chemical shifts are anisotropic and change with temperature.
