Skip to main content
NCBI home page
Springer Nature - PMC COVID-19 Collection logoLink to Springer Nature - PMC COVID-19 Collection
. 2006;581:297–300. doi: 10.1007/978-0-387-33012-9_51

Subcellular Localization of SARS-CoV Structural Proteins

Lisa A Lopez 3, Ariel Jones 4, William D Arndt 5, Brenda G Hogue 6
Editors: Stanley Perlman1, Kathryn V Holmes2
PMCID: PMC4524784  NIHMSID: NIHMS31943  PMID: 17037547

The content is available as a PDF (878.8 KB).

Contributor Information

Stanley Perlman, Email: Stanley-Perlman@uiowa.edu

Kathryn V. Holmes, Email: Kathryn.Holmes@ucHSC.edu

References

  • 1.Holmes KV, Lai MM. In: Fields Virology. 4. Fields BN, Knipe DN, Howley PM, editors. Philadelphia: Lippincott-Raven Publishers; 2001. [Google Scholar]
  • 2.Kuo L, Masters PS. The small envelope protein E is not essential for murine coronavirus replication. J. Virol. 2003;77:4597–4608. doi: 10.1128/JVI.77.8.4597-4608.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Vennema H, Godeke GJ, Rossen JW, Voorhout WF, Horzinek MC, Opstelten DJ, Rottier PJ. Nucleocapsid-independent assembly of coronavirus-like particles by co-expression of viral envelope protein genes. EMBO J. 2000;15:2020–2028. doi: 10.1002/j.1460-2075.1996.tb00553.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Baudoux P, Carrat C, Besnardeau L, Charley B, Laude H. Coronavirus pseudoparticles formed with recombinant M and E proteins induce alpha interferon synthesis by leukocytes. J. Virol. 1998;72:8636–8643. doi: 10.1128/jvi.72.11.8636-8643.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Corse E, Machamer CE. Infectious bronchitis virus E protein is targeted to the Golgi complex and directs release of virus-like particles. J. Virol. 2000;74:4319–4326. doi: 10.1128/JVI.74.9.4319-4326.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Escors D, Ortego J, Laude H, Enjuanes L. The membrane M protein carboxy terminus binds to transmissible gastroenteritis coronavirus core and contributes to core stability. J. Virol. 2001;75:1312–1324. doi: 10.1128/JVI.75.3.1312-1324.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Narayanan K, Kim KH, Makino S. Characterization of N protein self-associations in coronavirus ribonucleoprotein complexes. Virus Res. 2003;98:131–140. doi: 10.1016/j.virusres.2003.08.021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Almazan F, Galan C, Enjuanes L. The nucleoprotein is required for efficient coronavirus genome replication. J. Virol. 2004;78:12683–12688. doi: 10.1128/JVI.78.22.12683-12688.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Schelle B, Karl N, Ludewig B, Siddell SG, Thiel V. Selective replication of coronavirus genomes that express nucleocapsid protein. J. Virol. 2005;79:6620–6630. doi: 10.1128/JVI.79.11.6620-6630.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Niwa H, Yamamura K, Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991;108:193–199. doi: 10.1016/0378-1119(91)90434-D. [DOI] [PubMed] [Google Scholar]
  • 11.Fuerst TR, Niles EG, Studier FW, Moss B. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA. 1986;83:8122–8126. doi: 10.1073/pnas.83.21.8122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Nal B, Chan C, Kien F, Siu L, Tse J, Chu K, Kam J, Staropoli I, Crescenzo-Chaigne B, Escriou N, vander Werf S, Yuen K-Y, Altmeyer R. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. J. Gen. Virol. 2005;86:1423–1434. doi: 10.1099/vir.0.80671-0. [DOI] [PubMed] [Google Scholar]
  • 13.Lontok E, Corse E, Machamer CE. Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. J. Virol. 2004;78:5913–5922. doi: 10.1128/JVI.78.11.5913-5922.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Wurm T, Chen H, Hodgson T, Britton P, Brooks G, Hiscox JA. Localization to the nucleolus is a common feature of coronavirus nucleoproteins, and the protein may disrupt host cell division. J. Virol. 2001;75:9345–9356. doi: 10.1128/JVI.75.19.9345-9356.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Chang MA, Lu YT, Ho ST, Wu CC, Wei TY, Chen CJ, Hsu YT, Chu PC, Chen CH, Chu JM, Jan YL, Hung CC, Fan CC, Yang YC. Antibody detection of SARS-CoV spike and nucleocapsid protein. Biochem. Biophys. Res. Commun. 2004;314:931–936. doi: 10.1016/j.bbrc.2003.12.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Zeng R, Ruan HQ, Jiang XS, Zhou H, Shi L, Zhang L, Sheng QH, Tu Q, Xia QC, Wu JR. Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectrometric analysis. J. Proteome Res. 2004;3:549–555. doi: 10.1021/pr034111j. [DOI] [PubMed] [Google Scholar]
  • 17.Surjit M, Kumar R, Mishra RN, Reddy MK, Chow VT, Lal SK. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation. J. Virol. 2005;79:11476–11486. doi: 10.1128/JVI.79.17.11476-11486.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Rowland RR, Chauhan V, Fang Y, Pekosz A, Kerrigan M, Burton MD. Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in Vero cells. J. Virol. 2005;79:11507–11512. doi: 10.1128/JVI.79.17.11507-11512.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Nidoviruses are provided here courtesy of Nature Publishing Group

RESOURCES