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. Author manuscript; available in PMC: 2016 Jan 23.
Published in final edited form as: Nature. 2015 Jun 22;523(7561):472–476. doi: 10.1038/nature14449

Extended Data Figure 2. The effects of exogenous and endogenous SA on TOC1 expression.

Extended Data Figure 2

a, Luciferase activity measurements using the TOC1p:LUC plant extracts. Relative luciferase activity of the fifth and sixth leaves from 3-week-old soil-grown TOC1p:LUC plants. Water (CK) or 1 mM SA was applied at ZT24. LL, constant light. a.u., arbitrary unit. Data are mean ± s.e.m. (n = 6 biological replicates; t-test; ***, P < 0.001). b, TOC1p:LUC activity rhythms in 3-week-old soil-grown wild-type (WT) and sid2 plants treated with water (CK) or 1 mM SA at subjective dusk (black arrow) (mean ± s.e.m., n = 8 plants). White bars represent subjective days and grey bars represent subjective nights. The bar graphs represent the estimates of amplitude and average expression of TOC1p:LUC, respectively (mean ± s.e.m.). The letters above the bars indicate statistically significant differences between groups at P < 0.05 (Tukey’s multiple comparisons test). n.s., non-significant (two-way ANOVA, non-significant interaction between genotype and treatment). This experiment has been repeated three times with similar results.