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. 1991 Nov;10(11):3129–3136. doi: 10.1002/j.1460-2075.1991.tb04874.x

The three-dimensional structure of reovirus obtained by cryo-electron microscopy.

P Metcalf 1, M Cyrklaff 1, M Adrian 1
PMCID: PMC453034  PMID: 1915287

Abstract

The structures of reovirus serotypes T2J (Jones), T3D (Dearing) and the T3D core particle have been determined by cryo-electron microscopy and image processing. At a resolution of 30 A the two serotypes have similar features. The core is visible within the virus structure. The outer surface of the virus particles contains 120 holes at T = 13.1 local 6-fold axes. The holes penetrate into the virus as far as the surface of the internal core shell. Protrusions extending 4 nm from the virus surface surround each hole on the outside of the virus. At the 5-fold axes on the surface of the virus flat 'penton craters' form covers over the underlying core spikes. The detailed structure of the reovirus shell is very different to that of rotavirus although both have holes at T = 13.1 axes. Little evidence was seen of reovirus fibres extending from the virus surface.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian M., Dubochet J., Lepault J., McDowall A. W. Cryo-electron microscopy of viruses. Nature. 1984 Mar 1;308(5954):32–36. doi: 10.1038/308032a0. [DOI] [PubMed] [Google Scholar]
  2. Baker T. S., Drak J., Bina M. Reconstruction of the three-dimensional structure of simian virus 40 and visualization of the chromatin core. Proc Natl Acad Sci U S A. 1988 Jan;85(2):422–426. doi: 10.1073/pnas.85.2.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartlett N. M., Gillies S. C., Bullivant S., Bellamy A. R. Electron microscopy study of reovirus reaction cores. J Virol. 1974 Aug;14(2):315–326. doi: 10.1128/jvi.14.2.315-326.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bellamy A. R., Both G. W. Molecular biology of rotaviruses. Adv Virus Res. 1990;38:1–43. doi: 10.1016/s0065-3527(08)60858-1. [DOI] [PubMed] [Google Scholar]
  5. Bellamy A. R., Harvey J. D. Biophysical studies of reovirus type 3. III. A laser light-scattering study of the RNA transcriptase reaction. Virology. 1976 Mar;70(1):28–36. doi: 10.1016/0042-6822(76)90233-6. [DOI] [PubMed] [Google Scholar]
  6. Bican P., Cohen J., Charpilienne A., Scherrer R. Purification and characterization of bovine rotavirus cores. J Virol. 1982 Sep;43(3):1113–1117. doi: 10.1128/jvi.43.3.1113-1117.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Booy F. P., Newcomb W. W., Trus B. L., Brown J. C., Baker T. S., Steven A. C. Liquid-crystalline, phage-like packing of encapsidated DNA in herpes simplex virus. Cell. 1991 Mar 8;64(5):1007–1015. doi: 10.1016/0092-8674(91)90324-r. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crowther R. A. Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs. Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):221–230. doi: 10.1098/rstb.1971.0054. [DOI] [PubMed] [Google Scholar]
  9. Fraser R. D., Furlong D. B., Trus B. L., Nibert M. L., Fields B. N., Steven A. C. Molecular structure of the cell-attachment protein of reovirus: correlation of computer-processed electron micrographs with sequence-based predictions. J Virol. 1990 Jun;64(6):2990–3000. doi: 10.1128/jvi.64.6.2990-3000.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fuller S. D. The T=4 envelope of Sindbis virus is organized by interactions with a complementary T=3 capsid. Cell. 1987 Mar 27;48(6):923–934. doi: 10.1016/0092-8674(87)90701-x. [DOI] [PubMed] [Google Scholar]
  11. Furlong D. B., Nibert M. L., Fields B. N. Sigma 1 protein of mammalian reoviruses extends from the surfaces of viral particles. J Virol. 1988 Jan;62(1):246–256. doi: 10.1128/jvi.62.1.246-256.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harvey J. D., Bellamy A. R., Earnshaw W. C., Schutt C. Biophysical studies of reovirus type 3. IV. Low-angle x-ray diffraction studies. Virology. 1981 Jul 15;112(1):240–249. doi: 10.1016/0042-6822(81)90629-2. [DOI] [PubMed] [Google Scholar]
  13. Jayasuriya A. K., Nibert M. L., Fields B. N. Complete nucleotide sequence of the M2 gene segment of reovirus type 3 dearing and analysis of its protein product mu 1. Virology. 1988 Apr;163(2):591–602. doi: 10.1016/0042-6822(88)90300-5. [DOI] [PubMed] [Google Scholar]
  14. Joklik W. K. Studies on the effect of chymotrypsin on reovirions. Virology. 1972 Sep;49(3):700–715. doi: 10.1016/0042-6822(72)90527-2. [DOI] [PubMed] [Google Scholar]
  15. Kapuler A. M. An extraordinary temperature dependence of the reovirus transcriptase. Biochemistry. 1970 Oct 27;9(22):4453–4457. doi: 10.1021/bi00824a029. [DOI] [PubMed] [Google Scholar]
  16. Luftig R. B., Kilham S. S., Hay A. J., Zweerink H. J., Joklik W. K. An ultrastructural study of virions and cores of reovirus type 3. Virology. 1972 Apr;48(1):170–181. doi: 10.1016/0042-6822(72)90124-9. [DOI] [PubMed] [Google Scholar]
  17. Metcalf P. The symmetry of the reovirus outer shell. J Ultrastruct Res. 1982 Mar;78(3):292–301. doi: 10.1016/s0022-5320(82)80004-x. [DOI] [PubMed] [Google Scholar]
  18. Prasad B. V., Burns J. W., Marietta E., Estes M. K., Chiu W. Localization of VP4 neutralization sites in rotavirus by three-dimensional cryo-electron microscopy. Nature. 1990 Feb 1;343(6257):476–479. doi: 10.1038/343476a0. [DOI] [PubMed] [Google Scholar]
  19. Prasad B. V., Wang G. J., Clerx J. P., Chiu W. Three-dimensional structure of rotavirus. J Mol Biol. 1988 Jan 20;199(2):269–275. doi: 10.1016/0022-2836(88)90313-0. [DOI] [PubMed] [Google Scholar]
  20. Ralph S. J., Harvey J. D., Bellamy A. R. Subunit structure of the reovirus spike. J Virol. 1980 Dec;36(3):894–896. doi: 10.1128/jvi.36.3.894-896.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  23. Yeager M., Dryden K. A., Olson N. H., Greenberg H. B., Baker T. S. Three-dimensional structure of rhesus rotavirus by cryoelectron microscopy and image reconstruction. J Cell Biol. 1990 Jun;110(6):2133–2144. doi: 10.1083/jcb.110.6.2133. [DOI] [PMC free article] [PubMed] [Google Scholar]

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