Table 2.

Sequences of shRNA used to deplete the corresponding targets, and primers used for real time PCR

pLKO-1 shRNA IRF-1 target sequences
IRF-1	CGTGTGGATCTTGCCACATTT
IRF-1	CCTCTGTCTATGGAGACTTTA
IRF-1	GCAGATTAATTCCAACCAAAT
IRF-1	AGATGCTAAGAGCAAGGCCAA
IRF-1	GCGTGTCTTCACAGATCTGAA
TetOn-pLKO-1 shRNA
hiNOS	Forward: 5′-CCGGAACATTGCTGTGCTCCATAGTCTCGAGACTATGGAGCACAGCAATGTTTTTTG-3'
hiNOS	Reverse: 5′-AATTCAAAAAAACATTGCTGTGCTCCATAGTCTCGAGACTATGGAGCACAGCAATGTT-3'
GFP	Forward: 5′-CCGGCAGCCACAACGTCTATATCATCTCGAGATGATATAGACGTTGTGGCTGTTTTTG-3'
GFP	Reverse: 5′-AATTCAAAAACAGCCACAACGTCTATATCATCTCGAGATGATATAGACGTTGTGGCTG-3'
Real time PCR primers
hGAPDH	Forward: 5′-GTGAAGGTCGGAGTCAACGG-3′
	Reverse: 5′-AGTGATGGCATGGACTGTGG-3′
hiNOS	Forward: 5′-ACAACAAATTCAGGTACGCTGTG-3′
	Reverse: 5′-TCTGATCAATGTCATGAGCAAAGG-3′
IκBα	Forward: 5′-TGTCTACACTTAGCCTCTATC-3′
	Reverse: 5′-TCTGTGAACTCCGTGAACTC-3′
IRF-1	Forward: 5′-GAACTCCCTGCCAGATATCGAG-3′
	Reverse: 5′-TGCTCTTAGCATCTCGGCTGG-3′
SOCS1	Forward: 5′-TTTTTCGCCCTTAGCGTGA-3′
	Reverse: 5′-AGCAGCTCGAAGAGGCAGTC-3′
Tax	Forward: 5′-CGGATACCCAGTCTACGTGT-3′
	Reverse: 5′-GAGCCGATAACGCGTCCATCGATG-3′