FIG 2 .
Dose-dependent delivery of βlac by βlac-LTIIa. (A) Rat primary cortical neurons were incubated with 40 nM βlac-LTIIa at 37°C for 60 min. Cells were incubated with CCF2-AM at RT for 30 min followed by IF to detect LTIIa bound (anti-HA, red) and βlac (anti-FLAG, magenta). Uncleaved CCF2 is shown in green, and cleaved CCF2 (CCF2C) is shown in cyan. (B) Rat primary cortical neurons were incubated with 40 nM LTIIa or 0.1 to 40 nM βlac-LTIIa at 37°C for 60 min. Cleavage of CCF2 was quantified using the ratio of fluorescence of cleaved CCF2 to that of CCF2 as a function of added βlac-LTIIa.