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Delivery and separation of βlac from the B subunit during βlac-LTIIa entry into neurons. (A) Rat primary cortical neurons were incubated with 40 nM βlac-LTIIa at 4°C or 37°C for 60 min. Cells were washed, followed by IF staining, using anti-HA antibody (green) and anti-FLAG antibody (red). (B) Representative colocalization between HA and FLAG staining was shown by a cytofluorogram with Pearson’s coefficient (PC) determined. Bar, 20 µm.
