FIG 3 .

The activity of Bald’s eyesalve against S. aureus biofilms requires several ingredients. Two hundred microliters of the onion variant of the eyesalve (ES-O), the leek variant of the eyesalve (ES-L) (batch C), or preparations missing a single ingredient were added to three 1-day-old cultures of S. aureus in synthetic wounds, and bactericidal activity quantified as described in Fig. 2. Red bars show the results for the full or reduced versions of ES-O, blue bars show the results for the full or reduced versions of ES-L, and the green bar shows the result for a variant of the eyesalve with neither onion nor leek (ES). The control treatment (grey bar) was sterile distilled water. Individual ingredients dropped out of the recipe (Minus) are coded as follows: O, onion; L, leek; X, oxgall; W, wine; G, garlic; B, brass. Reduced recipes were assessed as having full (F), partial (P), or no (N) activity in comparison with the results for the control treatment and the appropriate complete recipe.