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. 1988 Jan;7(1):155–159. doi: 10.1002/j.1460-2075.1988.tb02795.x

Characterization of the hormone-binding domain of the chicken c-erbA/thyroid hormone receptor protein.

A Muñoz 1, M Zenke 1, U Gehring 1, J Sap 1, H Beug 1, B Vennström 1
PMCID: PMC454236  PMID: 3359993

Abstract

To identify and characterize the hormone-binding domain of the thyroid hormone receptor, we analyzed the ligand-binding capacities of proteins representing chimeras between the normal receptor and P75gag-v-erbA, the retrovirus-encoded form deficient in binding ligand. Our results show that several mutations present in the carboxy-terminal half of P75gag-v-erbA co-operate in abolishing hormone binding, and that the ligand-binding domain resides in a position analogous to that of steroid receptors. Furthermore, a point mutation that is located between the putative DNA and ligand-binding domains of P75gag-v-erbA and that renders it biologically inactive fails to affect hormone binding by the c-erbA protein. These results suggest that the mutation changed the ability of P75gag-v-erbA to affect transcription since it also had no effect on DNA binding. Our data also suggest that hormone-independent activity of P75gag-v-erbA provided a selective advantage to the avian erythroblastosis virus during the original selection for a highly oncogenic strain of the virus.

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Selected References

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