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. 2015 May 6;64(9):3111–3120. doi: 10.2337/db14-1627

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© 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

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Maternal HF feeding reduced expression of SIRT1, which suppresses LPL expression in trophoblasts. Western blotting and real-time PCR demonstrated remarkably reduced SIRT1 protein (A) and mRNA (B) levels in placental samples of E18.5 HF-fed dams (n = 6). Compared with WT MEFs, significantly higher LPL mRNA levels were found in Sirt1^−/− MEFs (n = 6) (C). Confluent JEG-3 trophoblasts were treated with SIRT1 activator RSV (10 μmol/L) or inhibitor EX-527 (1 μmol/L) overnight (13 h). EX-527 treatment robustly increased LPL protein levels and activities (D and E). In contrast, significantly reduced LPL protein levels and activities were found in RSV-treated trophoblasts (n = 6) (D and E). *P < 0.05 vs. control. Con, control.