Abstract
Characteristic chemiluminescence emission of singlet (1 delta g) molecular oxygen at 1268 nm is reported from a Haber-Weiss reaction. The reaction consists of mixing aqueous hydrogen peroxide with a solution of potassium superoxide, solubilized by 18-crown-6 ether in carbon tetrachloride or in dry acetonitrile at room temperature. Since the discovery of the enzyme superoxide dismutase by J.M. McCord and I. Fridovich [(1968) J. Biol. Chem. 243, 5733-5760], the identity of the reactive oxidant in superoxide-generating systems in biology has remained a chemical mystery. The results presented here suggest strongly that the reactive species is singlet oxygen generated via the Haber-Weiss reaction and not, as usually assumed, the hydroxyl radical, .OH, generated by the same reaction.
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Selected References
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- Beauchamp C., Fridovich I. A mechanism for the production of ethylene from methional. The generation of the hydroxyl radical by xanthine oxidase. J Biol Chem. 1970 Sep 25;245(18):4641–4646. [PubMed] [Google Scholar]
- Corey E. J., Mehrotra M. M., Khan A. U. Antiarthritic gold compounds effectively quench electronically excited singlet oxygen. Science. 1987 Apr 3;236(4797):68–69. doi: 10.1126/science.3563489. [DOI] [PubMed] [Google Scholar]
- Corey E. J., Mehrotra M. M., Khan A. U. Water induced dismutation of superoxide anion generates singlet molecular oxygen. Biochem Biophys Res Commun. 1987 Jun 15;145(2):842–846. doi: 10.1016/0006-291x(87)91041-2. [DOI] [PubMed] [Google Scholar]
- Khan A. U. Near infrared emission of singlet oxygen generated in the dark. J Biolumin Chemilumin. 1989 Jul;4(1):200–207. doi: 10.1002/bio.1170040129. [DOI] [PubMed] [Google Scholar]
- Khan A. U. Singlet molecular oxygen from superoxide anion and sensitized fluorescence of organic molecules. Science. 1970 Apr 24;168(3930):476–477. doi: 10.1126/science.168.3930.476. [DOI] [PubMed] [Google Scholar]
- McCord J. M., Fridovich I. The reduction of cytochrome c by milk xanthine oxidase. J Biol Chem. 1968 Nov 10;243(21):5753–5760. [PubMed] [Google Scholar]
- Steinbeck M. J., Khan A. U., Karnovsky M. J. Extracellular production of singlet oxygen by stimulated macrophages quantified using 9,10-diphenylanthracene and perylene in a polystyrene film. J Biol Chem. 1993 Jul 25;268(21):15649–15654. [PubMed] [Google Scholar]
- Steinbeck M. J., Khan A. U., Karnovsky M. J. Intracellular singlet oxygen generation by phagocytosing neutrophils in response to particles coated with a chemical trap. J Biol Chem. 1992 Jul 5;267(19):13425–13433. [PubMed] [Google Scholar]