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. 1988 Apr;7(4):899–904. doi: 10.1002/j.1460-2075.1988.tb02894.x

Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development

William E Gardiner 1,2, Garry Sunter 1,2, Leslie Brand 1, J Scott Elmer 1, Stephen G Rogers 1, David M Bisaro 1,2
PMCID: PMC454414  PMID: 16453835

Abstract

The geminiviruses are a unique group of higher plant viruses that are composed of twin isometric particles which contain circular, single-stranded DNA. Tomato golden mosaic virus (TGMV), a whitefly-transmitted agent, belongs to the subgroup of geminiviruses whose members possess a bipartite genome. The TGMV A genome component has the capacity to encode at least four proteins. One of these is the viral coat protein, as inferred by homology with coat-protein, genes of other geminiviruses and by the observation of typical geminate particles in transgenic plants that contain inserts of TGMV A DNA. We have investigated the role of the coat protein in TGMV replication and report here that its coding sequence may be interrupted or substantially deleted without loss of infectivity. However, certain coat-protein mutants showed reproducible delays in time of symptom appearance as well as reduced symptom development, when inoculated onto transgenic Nicotiana benthamiana plants containing the TGMV B component. The most attenuated symptoms were seen with a mutant in which the coat-protein coding sequence was almost entirely deleted. The significance of these findings for the development of plant vectors from TGMV DNA is discussed.

Keywords: geminivirus, Ti plasmid, agroinfection, plant vectors

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bisaro D. M., Hamilton W. D., Coutts R. H., Buck K. W. Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus. Nucleic Acids Res. 1982 Aug 25;10(16):4913–4922. doi: 10.1093/nar/10.16.4913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Daubert S., Shepherd R. J., Gardner R. C. Insertional mutagenesis of the cauliflower mosaic virus genome. Gene. 1983 Nov;25(2-3):201–208. doi: 10.1016/0378-1119(83)90224-x. [DOI] [PubMed] [Google Scholar]
  3. Gay N. J., Walker J. E. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase. Nature. 1983 Jan 20;301(5897):262–264. doi: 10.1038/301262a0. [DOI] [PubMed] [Google Scholar]
  4. Grimsley N., Hohn B., Hohn T., Walden R. "Agroinfection," an alternative route for viral infection of plants by using the Ti plasmid. Proc Natl Acad Sci U S A. 1986 May;83(10):3282–3286. doi: 10.1073/pnas.83.10.3282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hamilton W. D., Bisaro D. M., Buck K. W. Identification of novel DNA forms in tomato golden mosaic virus infected tissue. Evidence for a two component viral genome. Nucleic Acids Res. 1982 Aug 25;10(16):4901–4912. doi: 10.1093/nar/10.16.4901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamilton W. D., Bisaro D. M., Coutts R. H., Buck K. W. Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components. Nucleic Acids Res. 1983 Nov 11;11(21):7387–7396. doi: 10.1093/nar/11.21.7387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamilton W. D., Stein V. E., Coutts R. H., Buck K. W. Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J. 1984 Sep;3(9):2197–2205. doi: 10.1002/j.1460-2075.1984.tb02114.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howarth A. J., Caton J., Bossert M., Goodman R. M. Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3572–3576. doi: 10.1073/pnas.82.11.3572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kikuno R., Toh H., Hayashida H., Miyata T. Sequence similarity between putative gene products of geminiviral DNAs. Nature. 1984 Apr 5;308(5959):562–562. doi: 10.1038/308562a0. [DOI] [PubMed] [Google Scholar]
  10. Kunkel T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. doi: 10.1073/pnas.82.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MacDowell S. W., Coutts R. H., Buck K. W. Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus. Nucleic Acids Res. 1986 Oct 24;14(20):7967–7984. doi: 10.1093/nar/14.20.7967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rogers S. G., Bisaro D. M., Horsch R. B., Fraley R. T., Hoffmann N. L., Brand L., Elmer J. S., Lloyd A. M. Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants. Cell. 1986 May 23;45(4):593–600. doi: 10.1016/0092-8674(86)90291-6. [DOI] [PubMed] [Google Scholar]
  14. Stanley J. The molecular biology of geminiviruses. Adv Virus Res. 1985;30:139–177. doi: 10.1016/s0065-3527(08)60450-9. [DOI] [PubMed] [Google Scholar]
  15. Stanley J., Townsend R. Characterisation of DNA forms associated with cassava latent virus infection. Nucleic Acids Res. 1985 Apr 11;13(7):2189–2206. doi: 10.1093/nar/13.7.2189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stanley J., Townsend R. Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome. Nucleic Acids Res. 1986 Aug 11;14(15):5981–5998. doi: 10.1093/nar/14.15.5981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takamatsu N., Ishikawa M., Meshi T., Okada Y. Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA. EMBO J. 1987 Feb;6(2):307–311. doi: 10.1002/j.1460-2075.1987.tb04755.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Townsend R., Stanley J., Curson S. J., Short M. N. Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO J. 1985 Jan;4(1):33–37. doi: 10.1002/j.1460-2075.1985.tb02313.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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