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. 1988 Oct;7(10):2953–2960. doi: 10.1002/j.1460-2075.1988.tb03157.x

The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays

Heinrich Cuypers 1, Sudhansu Dash 1, Peter A Peterson 1, Heinz Saedler 1, Alfons Gierl 1
PMCID: PMC454676  PMID: 15977321

Abstract

Genetic and molecular analysis has revealed a specific En-element deletion derivative (En-I102) which reduces En/Spm-induced mutability. In the presence of En-I102 the excision frequency of both the autonomous En-1 element and the inhibitor element Spm-I5719A is reduced and excision occurs later in development. The 3697 bp long En-I102 element is derived from En-1 by an internal deletion of 4590 bp removing nucleotides 1862-6451. The promoter at the left end and sequences required for polyadenylation are retained in En-I102. It is transcribed to yield predominantly a 1.8 kb poly(A) RNA. cDNA analysis of this transcript indicated that it contains the coding capacity for a 386 amino acid polypeptide. This polypeptide shares homology with En/Spm encoded functions and we suggest that it interferes with transposition at the protein level.

Keywords: excision, gene structure, transposable element, Zea mays

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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