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. 1988 Oct;7(10):2961–2966. doi: 10.1002/j.1460-2075.1988.tb03158.x

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A L Schwartz 1, A Ciechanover 1, R A Brandt 1, H J Geuze 1
PMCID: PMC454677  PMID: 2846272

Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

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Selected References

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